Bacterial genomic DNA was extracted from pure culture with the fast spin kit (Invitrogen) following the manufacturer's instructions. Amplification of 16S rRNA gene was performed in 50 μL of reaction ...

2nd Department of General Surgery, Jagiellonian University Medical College, Krakow, Poland Department of Endoscopy, Jagiellonian University Medical College, Krakow, Poland Internal Diseases and ...

The urea breath test is used to detect Helicobacter pylori (H. pylori), a type of bacteria that may infect the stomach and is a main cause of ulcers in both the stomach and duodenum (the first part of ...

Preparation for the breath test involves abstaining from certain medications for several weeks and fasting food and water a few hours before the test. The test is safe during pregnancy and for ...

Quantifying urease activity is an important task for Microbial Induced Calcite Precipitation research. A new urease activity microplate assay using a fluorescent pH indicator is presented. The method ...

Calcium carbonate precipitation and crystallization induced by urease enzyme to solidify soil is known as biocement technology. The uses of waste and cheap materials can make this technology more cost ...

Several enzymes catalyze much of the processes that exist in the soil. Enzymes in polluted soils are usually less active due to their exposure to heavy metals. The main goal of this study was to see ...

The Supporting Information is available free of charge at https://pubs.acs.org/doi/10.1021/acs.analchem.1c00663. (Figures S1–S10) Structural and color changes ...

A paper based litmus test has been developed using modulation of urease enzyme activity for detection of C–C mismatch single nucleotide polymorphisms (SNPs) by the naked eye. Urease is first ...

Our work provides insights into how cells solve the problem of delivering nickel, a toxic metal, to the active site of a metalloenzyme such as urease. Urease, a nickel-containing enzyme, is a ...